RTG-ReMPro
Projects
P6 Jabs
Functional characterization of localization and interactions
of the Golgi-resident membrane protein LYSET
Objectives
We aim to understand i) if proteins other than GNPT are regulated by LYSET, ii) how LYSET is retained in the Golgi apparatus, and iii) whether posttranslational modifications such as e.g. methylation play a role in LYSET retention and interaction.
Aim i) Identification of other LYSET clients
Aim ii) Retention of LYSET in the Golgi apparatus
Aim iii) Furthermore, putative methylation sites will be identified by mutagenesis of arginine residues and IP using methyl-arginine specific antibodies
Functional characterization of localization and interactions of the Golgi-resident membrane protein LYSET A) GOLPH3/3L deficiency results in loss of LYSET from the Golgi apparatus, GNPT degradation and lysosomal dysfunction; B) consequences of interrupting GOLPH3/3L-LYSET interaction by posttranslational modifications (PTMs), e.g. dimethylation (Me2); C) consequences of inhibiting GNPT-LYSET interaction by PTMs.
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